Fully Human Antibody Discovery Using Nanovials and Plasma Cells From ATX-GKTM Mice
Thursday, Dec 7th, 2023
9 am PST/12 pm EST
The process of antibody discovery involves creating a vast array of potential candidates, aiming to retrieve as many of these candidates as feasible, and then assessing and screening them to pinpoint the most viable and promising ones. Recovering the antibodies from immunized animals typically involves mining the memory B cell compartment to retrieve variable region sequences of antigen-specific antibodies. An alternative and equally important source of antibody genes includes antibody-secreting plasma cells in the bone marrow and secondary lymphoid organs. Plasma cells, however, display low levels or do not display immunoglobulins on the cell surface which makes the detection and isolation of antigen-specific clones challenging. Existing technologies to screen antibody-secreting plasma cells require the use of specialized and expensive equipment and are limited in throughput. As such, pairing Alloy Therapeutics’ best-in-class transgenic humanized mouse with Partillion Biosciences’ Nanovial technology provides a simple and cost-efficient method to perform high throughput screens of antibody secreting plasma cells for the discovery of antigen-specific antibodies. In this webinar, we demonstrate an antibody discovery workflow that yields diverse, high affinity binders with development potential in therapeutic settings.
Learn how:
- ATX-Gx™ industry-leading humanized mouse models can be harnessed as in vivo human antibody discovery platform for generating a robust and diverse immune response
- Nanovial technology can screen hundreds of thousands of fragile antibody-secreting plasma without the need for expensive instrumentation or lengthy workflows.
- Plasma cells are a rich source of high affinity antibodies that have therapeutic potential
Speakers
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